Using the Spanish Verb Tratar

Utilizing the Spanish Verb 'Tratar' It may not appear to be coherent that a similar Spanish action word may intend to attempt and to treat, yet such is the situation with tratar. Tratar is a genuinely normal action word that additionally has different implications that dont appear to be especially related. When all is said in done, however, the word has to do with managing an individual, action, or thing. Frequently, the importance of tratar relies upon the relational word that follows, albeit even that isnt consistently a definite guide. Maybe more so than with most action words, you have to depend on the setting of the sentence to decide the significance. Regular Meanings of Tratar without anyone else One of the most run of the mill employments of tratar is to allude to rewarding a person or thing in a predefined way: Nos trataban como an animales y en ocasiones peor. (They rewarded us like creatures and now and then worse.)Siempre he estado en paz debido a la forma en que mi madre me tratã ³. (I have consistently found a sense of contentment due to the manner in which my mom rewarded me.) ¡No me trates como an un niã ±o! (Dont treat me as a youngster!) All the more explicitly, tratar is regularly used to allude to clinical treatment. La enfermedad de Lyme se trata con antibiã ³ticos. (Lymes malady is treated with antibiotics.)Los mã ©dicos van a tratar de detener la hemorragia con medicamentos. (The specialists are going utilize a treatment of halting the drain with medications.)Trataron el dolor con morfina por casi una semana. (They rewarded the torment with morphine for very nearly a week.)El hospicio trata al paciente como an una persona en su totalidad. (A hospice regards the patient as an individual in their totality.) Utilizing Tratar With De When tratar is trailed by the relational word de and an infinitive, it intended to attempt or endeavor: Aquã ­ no tratamos de ganar mentes y corazones, porque es irrelevante. (Here we are making an effort not to win hearts and psyches, since it is irrelevant.)Trate de nadar por lo menos una hora y quince minutos. (Attempt to swim at any rate an hour and 15 minutes.)Los participantes del estudio que fotografiaron sus alimentos perdieron ms peso que aquellos que simplemente trataron de comer ms sanamente. (The examination members who captured their food lost more weight than the individuals who basically attempted to eat more healthily.)Tratarà © de esturiar tasks los dã ­as que pueda excepto los lunes. (I will attempt to concentrate each day I can with the exception of Mondays.)Trata de ponerte en mis zapatos. (Attempt to imagine my perspective.) At the point when the reflexive structure tratarse is trailed by de, it demonstrates the subject of something or what its about. Fahrenheit 451 trata de una sociedad totalitaria donde los libros estn prohibidos. (Fahrenheit 451 is about an authoritarian culture where books are banned.)Se trata del coche de mi padre, doy una opiniã ³n de primera mano. (Regarding the matter of my dads vehicle, I am giving a firsthand opinion.) ¿De quã © trata? Whats it about?La vida se trata de contentamiento y gratitud. (Life is about happiness and appreciation.) Utilizing Tratar With Con When either tratar or tratarse is trailed by con, the relational word generally significance with, it can allude to a connection between at least two gatherings. Note that in certain unique circumstances, tratar con can rather me to treat with. Este cambio afectar la manera en que usted trata con su compa㠱ã ­a de telã ©fonos. (This change will influence the manner in which you work with your telephone company.)Con respecto al love, no me trato con gente city hall leader que yo. (With regards to sentiment, I dont have associations with individuals more seasoned than I am.)Sin ban, muchas veces se tratã ³ con à ©l. (In any case, I regularly had dealings with him.)Los compaã ±eros tratan con mucho respeto. (The colleagues treat each other with incredible regard.) Key Takeaways Tratar regularly intends to treat, as in rewarding a person or thing a specific way, or in giving clinical treatment.Tratar de when followed an infinitive for the most part intends to attempt or attempt.Tratarse de is utilized to show what something is about.

Immigration as Social Issue in Australia

Presentation Immigration is a prickly political just as social issue in Australia. In the same way as other western nations, Australia gets a huge number of outsiders from underdeveloped nations who enter the nation both legitimately and unlawfully. As indicated by the Australian government, the nation has gotten almost 6.8 million foreigners since 1945.Advertising We will compose a custom coursework test on Immigration as Social Issue in Australia explicitly for you for just $16.05 $11/page Learn More Middle East nations like Lebanon supplies the greater part of the settlers from the locale to Australia. Expanded limitations on movement on the scenery of a period ruled by against dread measures after 911 that have seen an awful crackdown and confinement of foreigners by Australian specialists. There have likewise been racially roused assaults of foreigners in Australia particularly those of Asian inception. As per an assessment of public sentiment did in 2005, most Australians were of the conclusion that less workers from the Middle East ought to be permitted into the nation. Mill operator et al (2009) says that in the midst of all these notwithstanding, is the fascinating issue of patriotism and character of these settlers particularly those from the Middle East (p. 208). A portion of these outsiders have procured Australian citizenship while numerous others are moving in the direction of accomplishing the Australian dream. That viably implies they are working for the Australian reason. It ought to never be overlooked anyway that these migrants have bind to their country and that is assumes a critical job in characterizing their patriotism and identity. Already accessible investigations by people, government, and non-administrative associations will be the wellspring of data. Examination and audit of this data to decide the patterns in it will include the procedure of the investigation. A lot of ends will be drawn from the examination of writing audit. Wher e essential, there will likewise be suggestions. The ends will be contrasted with the speculations with check whether the suppositions that educated the endeavor regarding the investigation were right or not. Generally pattern Nieuwenhuysen et al (2009) says that a difference in Australian movement approach in the 1970’s saw an expansion of non-Europeans particularly Asians and individuals from the Middle East in Australia (p. 98). This migration has offered ascend to another pattern of trans-patriotism that frames the premise of writing review.Advertising Looking for coursework on sociologies? We should check whether we can support you! Get your first paper with 15% OFF Learn More The exploration will investigate the component of trans-patriotism that is probably going to be the situation with the greater part of these workers and its effects on their proceeded with remain in Australia. Transnationalism will likewise incorporate an assessment of the parts of cultural assimil ation, absorption, and combination of these outsiders to the bigger Australian people group. There will be a top to bottom investigation and examination of the writing that exists on transnationalism. Research will concentrate on the common ties that middles East settlers have to their country and what it intends to their Australian and their local countries’ personality and patriotism. Different parts of transnationalism including government arrangements and race relations will likewise be considered and their effect on the patriotism and personality of these foreigners. These perspectives incorporate the effect of country legislative issues, devotion to Australia and their local terrains, citizenship obtaining and equivalent access to circumstances. Measures According to Soller (2004), writing survey will be isolated into different segments that will concentrate on the point under investigation (p.104). Audit of the status of Middle East workers in the nation, the example o f migration and settlement in Australia, surveys on the current writing on movement of Middle East individuals to Australia, synopses, and ends from the examination done on writing survey will cosmetics the rules for the writing audit. References Soller, A. J. (2004) Evaluation of microbial hazard appraisal procedures and applications. Alexandria: Water condition establishment.. Nieuwenhuysen et al. (2009) Nations of outsiders: Australia and the USA thought about. Cheltenham: Edward Elgar Publishing. Mill operator et al. (2009) Immigration to Australia. London: VDM Publishing House Ltd.Advertising We will compose a custom coursework test on Immigration as Social Issue in Australia explicitly for you for just $16.05 $11/page Learn More This coursework on Immigration as Social Issue in Australia was composed and put together by client Eleanor Rutledge to help you with your own examinations. You are allowed to utilize it for research and reference purposes so as to compose your own paper; be that as it may, you should refer to it appropriately. You can give your paper here.

A Guide to Acquiring Textbooks

A Guide to Acquiring Textbooks These days, college students are faced with an impossible task: purchasing textbooks. And with the purchase of textbooks comes a boatload of questions. Today, I hope to not only address the most common questions about purchasing textbooks, but smash some common misconceptions, too! 1. Where do I buy my textbooks? View this post on Instagram THE place to buy textbooks, school supplies, and your new favorite Illini apparel! A post shared by Illini Union Bookstore (@illiniunionbookstore) on Aug 25, 2014 at 2:00pm PDT Textbooks can be purchased at the Illini Union Bookstore, located on Wright Street. The Illini Union Bookstore is convenient for all students, offering pick-up, shipping, and returns. Students can search for books by campus term, department, course, and section. Students will also find University of Illinois merchandise for sale at the bookstore. The bookstore is open weekdays from 7:30 am 8:00 pm and weekends from 9:00 am 6:00 pm. 2. Should I buy my textbooks before classes start? Unfortunately, there is no clear-cut answer to this question, as it depends on the course. In my eyes, you can never be too prepared, so if you know you will use your textbook, the earlier the better. However, this is not the most cost-effective method. There is no guarantee that you will use every textbook you buy, or that every textbook listed on your course syllabus will be required. My best advice? Check in with your professor or teaching assistant to ask how the textbook is used in the course. 3. How much will it cost? The shorthand answer? Textbooks can be pricey. In fact, textbooks have wracked up quite a fee in my college spending. That being said, I have developed a few tips and tricks to making textbook-purchasing a more economic decision. First, buy the textbook used instead of new. Used textbooks are often in great condition and can save you tons of money. Second, check second-hand sellers, such as Amazon, for the same book. The bookstore is quick and easy, but may not always be the least expensive option. Again, Amazon offers books new and used. Chegg is also a great resource. In some cases, you may even share a textbook with a friend, which is a two-for-one deal! 4. Should I rent or should I buy? View this post on Instagram Happy New Year! #2020 A post shared by @ chegg on Jan 1, 2020 at 8:15am PST Unless you plan on heavily annotating your textbook, I would stick to renting. But like all things, it comes down to personal preference. Renting will save you tons of money when it comes to acquiring textbooks. The downside, of course, is that the book is not yours to own, and you are responsible for the books damage. Thinking of renting? Check out Chegg, Amazon, CampusBooks, and Kindle. And with this question comes an embarrassing story from my freshman year. Being the poor college freshman I was, I decided to rent a textbook through Chegg for a spring semester course. The spring semester came and went, and I gladly sped into my summer vacation. In July, I received a rude awakening. That textbook I had rented for $20 wracked up a $60 late fee, as I had never returned it. Moral or the story? Set a reminder on your phone to return your rented books well before the due date! Rachel Class of 2020 I am studying Middle Grades Education with concentrations in Social Sciences and Literacy in the College of Education. Although I now reside in Champaign, I am originally from Vernon Hills, a Northwest suburb of Chicago.

Should Athletes Use Performance Enhancing Drugs Help Push...

Introduction Attention Grabber: Depression, convulsions, anxiety, jaundice, myocardial infarction aka heart attack, eczema, abscess. All of these are listed in the FDA’s Adverse Event Reporting System as known side effects of Vitamin C(Adverse Event Reporting System)†¦? Huh...What makes steroids more controversial than Vitamin C? After all, it’s common knowledge that the abuse of pretty much anything can come with some pretty severe adverse effects. Thesis: Today I am going to tell you why athletes should be able to use performance-enhancing drugs to push themselves farther than their natural limits. Credibility: I feel as if I’m credible to speak on the topic because a good friend of mine provided a very fact driven argument on the topic when I ragged on him for taking the stuff. That argument led me to do a lot of research on the topic to try and prove him wrong and instead I just gained a lot of knowledge on the subject and ended up convincing myself that most of what I’ve ever heard about PEDs is simply wrong. Main Points: Today, I’m going to focus mostly on the health issues, because in my opinion, that’s the most important subject in this topic. I will also touch on the morality issues behind all of this. And I will tell you all who stands to gain from all the misinformation and propaganda passed around on this subject. Body 1st Main Point: Everything we see on TV tells us that steroids kill. But I’ve been told not to believe everything I see on TV. Do steroids reallyShow MoreRelatedDeveloping Management Skills404131 Words   |  1617 Pagesmymanagementlab is an online assessment and preparation solution for courses in Principles of Management, Human Resources, Strategy, and Organizational Behavior that helps you actively study and prepare material for class. Chapter-by-chapter activities, including built-in pretests and posttests, focus on what you need to learn and to review in order to succeed. Visit www.mymanagementlab.com to learn more. DEVELOPING MANAGEMENT SKILLS EIGHTH EDITION David A. Whetten BRIGHAM YOUNG UNIVERSITY Read MoreMarketing Mistakes and Successes175322 Words   |  702 Pageswritten permission of the Publisher, or authorization through payment of the appropriate per-copy fee to the Copyright Clearance Center, Inc. 222 Rosewood Drive, Danvers, MA 01923, website www.copyright.com. Requests to the Publisher for permission should be addressed to the Permissions Department, John Wiley Sons, Inc., 111 River Street, Hoboken, NJ 07030-5774, (201)748-6011, fax (201)748-6008, website http://www.wiley.com/go/permissions. To order books or for customer service please, call 1-800-CALLRead MoreHuman Resources Management150900 Words   |  604 PagesCHAPTER 1 Changing Nature of Human Resource Management After you have read this chapter, you should be able to: ââ€"  Identify four major HR challenges currently facing organizations and managers. List and define each of the seven major categories of HR activities. Identify the three different roles of HR management. Discuss the three dimensions associated with HR management as a strategic business contributor. Explain why HR professionals and operating managers must view HR management as anRead MoreStephen P. Robbins Timothy A. Judge (2011) Organizational Behaviour 15th Edition New Jersey: Prentice Hall393164 Words   |  1573 PagesHall. All rights reserved. Manufactured in the United States of America. This publication is protected by Copyright, and permission should be obtained from the publisher prior to any prohibited reproduction, storage in a retrieval system, or transmission in any form or by any means, electronic, mechanical, photocopying, recording, or likewise. To obtain permission(s) to use material from this work, please submit a written request to Pearson Education, Inc., Permissions Department, One Lake Street, Upper

Essay about The Picture of Dorian Gray by Oscar Wilde

The Picture of Dorian Gray by Oscar Wilde In the book, The Picture Of Dorian Gray by Oscar Wilde, there is a character named Lord Henry Wotton. He is the storys antagonist and whom critics often think most resembles Oscar Wilde. Wilde remarks Basil Hallward is what I think I am: Lord Henry what the world thinks of me: Dorian what I would like to be-in other ages perhaps. Within the preface of The Picture Of Dorian Gray, there lie the lines Those who go beneath the symbol do so at their peril. Those who read the symbol do so at their own peril. From Wildes statement, we can assume that there is a part of Wilde represented in each of the main characters, but how they represent him is up for the reader to decide. According†¦show more content†¦The reason I will not exhibit this picture is that I am afraid that I have shown in it the secret of my own soul. (23) Throughout the book, it is easily seen that Basil has an interest for Dorian. There are some homosexual characteristics in Basil but he is restricted by the Victorian society to ever act upon it. In this way, Oscar Wilde is represented in the character Basil Hallward. According to critics, Lord Henry Wotton most closely represents Wildes character. Critics see Wildes appreciation for all kinds of art as a form of Hedonism. The body sins once, and has done with its sin, for action is a mode of purification. Hedonism is what Lord Henry believes in; the belief that all that matters is seeking pleasure in life. Lord Henry is also seen as a bad influence and a very seductive person. Wilde writing this book is seen as a way of convincing the public to become hedonists and therefore critics compare him to Lord Henry. Finally, Wilde says that he would like to be Dorian but in another time. Dorian starts out as a handsome, wealthy gentleman thats innocent. He is quickly seduced by Lo rd Henrys words and starts to live life for all its pleasures. These acts are obviously not accepted by the public and with his picture taking all the flaws, his image is never damaged. In my opinion, I believe that when Wilde said he wants to be Dorian, its because he wishes that he could get rid of a temptation by yielding to it,Show MoreRelatedThe Picture Of Dorian Gray By Oscar Wilde2792 Words   |  12 Pages The Picture of Dorian Gray by Oscar Wilde In Oscar Wilde’s first novel The Picture of Dorian Gray, Wilde strategically uses his main characters Lord Henry Wotton and Basil Hallward to represent good versus evil influences throughout the reading. In the book, Dorian Gray plays the role of the everyman who is in a vicious circle on having to decide between the side of good or evil. Lord Henry is the evil influence and is seen as a more devil-like character while Basil Hallward is the good ChristianityRead MoreThe Picture Of Dorian Gray By Oscar Wilde1523 Words   |  7 PagesReader Response Entry #6: Chapters 10-11 The Picture of Dorian Gray by Oscar Wilde was not received well by critics when it was published in 1890. This was because it contained themes of homosexuality and was considered scandalous. Now, it is just considered a philosophical novel dealing with morals. I think that this book would very much be viewed as indecent in Wilde’s time. For example, when talking about Dorian’s public image, Wilde writes, â€Å"Society--civilized society, at least--is never veryRead MoreThe Picture of Dorian Gray by Oscar Wilde832 Words   |  3 Pagescharacteristics of self-destructive properties. In the novel The Picture of Dorian Gray, Oscar Wilde shows how these can lead to a man’s downfall. He displays this through the character of Dorian Gray. The novel explains how as Dorian grows up and through his life, he is ultimately destroyed by his own ego, vanity and inability to change or realize how what he does affects not only him but the lives of those around him. Dorian Gray struggles throughout the novel with the daunting facts that he isRead MoreThe Picture of Dorian Gray by Oscar Wilde1967 Words   |  8 Pages In The Picture of Dorian Gray, Oscar Wilde disputes the role and conflicts between Aestheticism and morality. He exposes his contradictions and inner struggles throughout his three main characters: Lord Henry, a nobleman who criticizes the moralism and hypocrisy of Victorian society and openly expresses his Aesthetic thoughts, Dorian Gray, a handsome model influenced by Lord Henry’s views on beauty and morality, and Basil Hallward, an artist captivated by Dorian’s beauty. The novel mainly dealsRead MoreThe Picture Of Dorian Gray By Oscar Wilde1414 Words   |  6 Pagesto me†¦(â€Å"Fallenoracle†).† This quote from the tv series Quantico does an effective job of expressing what Lord Henry’s influence has done to Dorian Gray throughout the philosophical novel, The Picture of Dorian Gray by Oscar Wilde. While some might argue that Dorian s actual nature does not change significantly, an additional perspective is that Dorian Gray is a dynamic char acter that gains negative character traits by becoming vain of his youth, increasingly paranoid of someone learning of his portraitRead MoreThe Picture Of Dorian Gray By Oscar Wilde1060 Words   |  5 PagesThe Picture of Dorian Gray by Oscar Wilde is a philosophical/gothic fiction. Setting: The novel takes place in the peak of the Decadent artistic movement of the 1890s, which occurred in the Victorian era of London and is known for its judgmental social standards, highlighting the contrast between the wealthier, materialistic higher classes and the dull middle-class society, making the novel more audacious. Plot: A famous artist named Basil Hallward completes his first portrait of Dorian Gray: aRead MoreThe Picture of Dorian Gray by Oscar Wilde687 Words   |  3 PagesThesis-In The Picture of Dorian Gray by Oscar Wilde, main character, Dorian Gray , in his times of greatest pain chooses to focus only on what is beautiful which leads to his death and shows that beauty obscures reality. Dorian Grays romantic interest, Sibyl kills herself after an altercation with Dorian; he looks at the nature around him to avoid the reality of the suicide that he has caused. Dorian first sees Sibyl when she acts at a rundown and low quality theater. She acts as many charactersRead MoreThe Picture Of Dorian Gray : Oscar Wilde2014 Words   |  9 PagesAdrian Balakumar Mr.Sal AP Lit 15 December 2014 The Picture of Dorian Gray - Oscar Wilde 1) In the book, The Picture of Dorian Gray, several characteristics of the world have been presented. The author portrays the world as a den of individuals with a skewed view of the immediate environment. The author depicts the world as a place where physical traits like beauty or handsomeness are important than a person’s virtues. Dorian spends his time studying music and other beautiful things that life hasRead MoreThe Picture Of Dorian Gray By Oscar Wilde1731 Words   |  7 PagesMy choice for this summer reading assignment was to read The Picture of Dorian Gray by Oscar Wilde. This is a philosophical fiction novel meaning it devotes a significant portion of the novel to sort the questions that are addressed in discursive philosophy, that can include: the role in society, the purpose of living, ethnic or morals, experience, etc. Throughout history art has played a huge role in portraying the structure of society and how the people play in it.The novel takes place in theRe ad MoreThe Influences of Oscar Wilde The Picture of Dorian Gray1582 Words   |  7 PagesThe Influences of Oscar Wilde Throughout his life Oscar Wilde had many strong influences exerted upon him. During his early childhood his mother influenced him and into college some of his professors and certain philosophers left a substantial impression upon him. Into adulthood these influences leaked out in his writing. These influences gave him ample ideas for writing The Picture of Dorian Gray. Wildes study of the Hellenistic ideals of Epicurus, his coddled lifestyle as a child and his devotion

Master of Business Administration program at Georgia State University Free Essays

I have chosen the flexible Master of Business Administration program at Georgia State University because it offers the best academic and skills training that would help me become better at what I do. At present I am interested in learning communication and analytical skills, how to be decisive and the importance of teamwork. I believe that these skills are necessary for the success of my career as a financial accountant and I know that I would be able to learn this through the challenging curriculum of Robinson College. We will write a custom essay sample on Master of Business Administration program at Georgia State University or any similar topic only for you Order Now Work experience is necessary for real life application, but education broadens one’s thinking and perspectives. My main reason for pursuing an MBA is because I want to further myself in the accounting field. Through the training of the MBA program I will be equipped with the skills that would allow me to help businesses expand in their operations while focusing on management and employee connections. Personally I believe that I have the leadership skills and the creative perspective for growth to be successful in my chosen career but I am also aware that I lack the educational background. By enrolling in the MBA program of Georgia State University and practicing my profession, I know that I would be able to become a financial advisor wherein I could help clients increase their revenues by using internal assessments and other strategies. I hope to become a financial accountant in the future and work on financial analysis and prepare fiscal reports. Through several work experiences I realized that I could do anything I set my mind to accomplish; that through hard work and determination I could rise from a party coordinator to manager. I also learned that a successful business enterprise is possible if there is honesty and complete customer satisfaction. I have had the opportunity to work as a Payroll and Tax Specialist, and here I have learned that customer satisfaction and the highest quality service is the best way to develop customer loyalty. All of these experiences have no doubt enriched my professional career but I also know that I need to learn more about this field of specialization and I am most happy when I am learning something new. I know that I belong to Georgia State’s MBA program; it has the right balance of academic and practical training that appeals to me. My contributions to the university will be my collective perspective, my determination to succeed and moral character. My degree in Bachelors in Family and Consumer Sciences has imbued me with the knowledge and sensitivity to understand people. By becoming a member of the MBA program I will bring with me this humanitarian perspective and help influence other business professionals to do the same. I am determined to finish this degree and I am prepared to give my best in accomplishing the requirements of the course and to actively participate in each class. The strong business ethics and professional accountability of Robinson College is the best place to foster and develop my skills and personality. How to cite Master of Business Administration program at Georgia State University, Papers

Nco Disrespect Towarsds Essay Sample free essay sample

Disrespecting An NCO Essay Today this essay will be about disrespecting a Non Commission Officer or ( NCO ) . There is many different ways and form’s of disrespecting an NCO. One manner that you could disrespect an NCO is the manner you talk. move. facial motions. how your voice is. your organic structure linguistic communication. and most of all how you present yourself towards an NCO. Lets start off with the manner you talk. The manner you talk can be a large factor in how your SGT can take it. So the best manner to avoid any confrontation between you and your SGT is to ever hold to what there stating. When they tell you something you do it. Its yes SGT Roger SGT. Its non good SGT. possibly SGT. or No SGT. You ever have to be respectful towards Your SGT because in the military its all about the superior system. We will write a custom essay sample on Nco Disrespect Towarsds Essay Sample or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page You may non esteem the individual but you have to esteem the rank. So you ever have to be respectfull when there speaking to you. Now the following thing that’s a large factor on being respectful towards a NCO is on how you move. When a NCO negotiations to you. You ever have to stand at the place of parade remainder. You have to stand up consecutive and with shoulder back. cervix back and when there speaking to you you have to look them directly in the oculus. Now the following thing to make to esteem NCO is your facial motions. When your NCO’S are speaking to you have to look them directly in the oculus without any facial motions. you cant cock your oculus brows you cant turn over your eyes. you have to reasonably much have no facial looks. You have to look them directly faced non laughting nil. And now one of the last things about esteeming NCO’S is the manner your voice is towards the NCO. When you talk to person like I said you have to be like yes sgt. no sgt. roger sgt. and when there speaking to you. You cant have that toan in your voice where it sounds like your seeking to revenge. you cant have the voice like your seeking to be better so your NCO. You have to cognize that you are non above he or she. Like me personally im merely a private so I don’t have a say so in how things work. I cant act like im better so the certain individual. Or it will consequence my hereafter and ruein me for the remainder of my life. So that’s why I try to be the most respectful I can be. But some times it is so difficult to make because I have that revenging image in me from when I was turning up I feel like I don’t need to be taken attention of and I don’t like people giving me counsel because I feel I can make it on my ain and if I don’t do it a certain manner I feel like I get in problem for no ground. I mean if I can make it a manner that’s works best for me and make the right manner I feel like it will work out. Now I believe this is the last thing about disrespecting NCO is how you present yourself towards a NCO.

A Memorable Event in My Life Essay Example

A Memorable Event in My Life Essay Example A Memorable Event in My Life Paper A Memorable Event in My Life Paper My Life Being stuck In one of the most remote places In Cambodia does not seem like part of a vacation plan for anyone. In our lives, there will always be times when things do not go as the way you planed. Due to not preparing for any emergencies, our family learned a very important lesson in life. I was 13 when this incident happened. My family and I were on holiday in Sandhog province when we were left praying for help as our car got caught in the wet and muddy soil on the border of Sandhog and Henna province. We had been driving for six hours when we reached Luminous. The stone that made up the road became dirt, buildings turned Into trees and cars turned Into cows. As we moved on, our surrounding became more and more remote. Soon there was no sight of any human being, only dirt roads stretched out like veins running through our bodies. The wind of the coming storm began to pick up speed. Trees swayed from side to side as the gloaming sky rumbled and the lighting flashed. I tried to control my fear but the sound of the storm seems to suppress my ability to control my emotions. Then the unthinking happened. Two of our back wheels were sucked by the muddy soil; It could no long propel us forward. We were stuck. Father stepped hard on the gas pedal over and over again but it was in vain. Trapping in the middle of nowhere turned my fear into tears. What happen if there were bandits? What happened if there was a landslide? I could not help but thought of all the possible ways of how I will die. This doesnt seem good, wave got to hurry. Fathers words seem to have woken me up from a dream. I was no longer crying. The fear of dying gave me strength. I got out of the car and with all of my strength, I pushed. However my effort yields no result. Then an Idea struck me. I went to the back of the car and grabbed the two paintings that I have bought in Kingdom and placed it under the back wheels. Again, we pushed. As I hoped and prayed, the car went over the painting and we were freed. Smiles followed by relieve were on everyones face. As e continued on with our Journey, the rain did not stop and the chances of us trapping In the muddy soil were still there but this time I was ready. I would not sit and Walt for father to save me. My fear had turned Into strength. For the rest of the journey we trapped in the muddy soil for three more times but we managed to overcome the obstacle as one. This incident made me realized that no matter what difficulties we faced, as long as we have the perseverance, determination and the willingness to work as one, we will be able to overcome it.

The Historic Olmec City of San Lorenzo

The Historic Olmec City of San Lorenzo The Olmec culture thrived along Mexico’s Gulf coast from roughly 1200 B.C. to 400 B.C. One of the most important archaeological sites associated with this culture is known as San Lorenzo. Once, there was a great city there. Its original name has been lost to time. Considered by some archaeologists to be the first true Mesoamerican city, San Lorenzo was a very important center of Olmec commerce, religion, and political power during its heyday. Location San Lorenzo is located in Veracruz State, about 38 miles (60km) from the Gulf of Mexico. The Olmecs could not have selected a better site to build their first great city. The site was originally a large island in the middle of the Coatzacoalcos River, although the course of the river has since changed and now only flows past one side of the site. The island featured a central ridge, high enough to escape any flooding. The floodplains along the river were very fertile. The location is close to sources of stone which were used for making sculptures and buildings. Between the river on either side and the high central ridge, the site was easily defended from enemy attack. Occupation of San Lorenzo San Lorenzo was first occupied around 1500 B.C., making it one of the oldest sites in the Americas. It was home to three early settlements, referred to as the Ojochà ­ (1500-1350 B.C.), the Bajà ­o (1350-1250 B.C.), and the Chichrras (1250-1150 B.C.). These three cultures are considered pre-Olmec and are largely identified by pottery types. The Chicharrs period begins to show characteristics later identified as Olmec. The city reached its peak in the period from 1150 to 900 B.C. before falling into decline. This is referred to as the San Lorenzo era. There may have been some 13,000 inhabitants at San Lorenzo during the height of its power (Cyphers). The city then went into decline and passed into the Nacaste period from 900 to 700 B.C. The Nacaste did not have the skills of their forebears and added little in the way of art and culture. The site was abandoned for some years before the Palangana era (600-400 B.C.). These later inhabitants contributed some small mounds and a ball cou rt. The site was then abandoned for over a thousand years before it was re-occupied during the Late Classic era of Mesoamerican civilization, but the city never regained its former glory. The Archaeological Site San Lorenzo is a sprawling site which includes not only the one-time metropolis of San Lorenzo but several smaller towns and agricultural settlements that were controlled by the city. There were important secondary settlements at Loma del Zapote, where the river forked to the south of the city, and El Remolino, where the waters re-converged to the north. The most important section of the site is on the ridge, where the nobility and priest classes lived. The western side of the ridge is known as the â€Å"royal compound,† as it was home to the ruling class. This area has yielded a treasure trove of artifacts, particularly sculptures. The ruins of an important structure, the â€Å"red palace,† are also found there. Other highlights include an aqueduct, interesting monuments scattered around the site, and several artificial pits known as â€Å"lagunas,† the purpose of which is still unclear. Stonework Very little of Olmec culture has survived to the present day. The climate of the steamy lowlands where they lived has destroyed any books, burial sites, and items of cloth or wood. The most important remnants of the Olmec culture are therefore architecture and sculpture. Fortunately for posterity, the Olmec were talented stonemasons. They were capable of transporting large sculptures and blocks of stone for masonry for distances of up to 60 kilometers (37 miles). The stones were probably floated part of the way on sturdy rafts. The aqueduct at San Lorenzo is a masterpiece of practical engineering. Hundreds of similarly-carved basalt troughs and covers weighing many tons were laid out in such a way as to promote the flow of water to its destination, which was a duck-shaped cistern designated Monument 9 by archaeologists. Sculpture The Olmec were great artists and the most remarkable feature of San Lorenzo is doubtless the several dozen sculptures that have been discovered at the site and nearby secondary sites like Loma del Zapote. The Olmec were famous for their detailed sculptures of colossal heads. Ten of these heads have been found at San Lorenzo. The largest of them is nearly ten feet tall. These massive stone heads are believed to depict rulers. At nearby Loma del Zapote, two finely sculpted, nearly identical twins face two jaguars. There are also several massive stone thrones at the site. All in all, dozens of sculptures have been found in and around San Lorenzo. Some of the statues were carved out of earlier works. Archaeologists believe that the statues were used as elements in scenes with religious or political meaning. The pieces would be laboriously moved around to create different scenes. Politics San Lorenzo was a powerful political center. As one of the first Mesoamerican cities - if not the first - it did not have true contemporary rivals and ruled over a large area. In the immediate environs, archaeologists have discovered many small settlements and dwellings, mostly located on hilltops. The smaller settlements were likely ruled by members or appointments of the royal family. Smaller sculptures have been found at these peripheral settlements, suggesting that they were sent there from San Lorenzo as a form of cultural or religious control. These smaller sites were used in the production of food and other resources and were of strategic use militarily. The royal family ruled this mini-empire from the heights of San Lorenzo. Decline and Importance In spite of its promising start, San Lorenzo fell into steep decline and by 900 B. C. was a shadow of its former self. The city would be abandoned a few generations later. Archaeologists do not really know why San Lorenzos glory faded so soon after its classic era. There are a few clues, however. Many of the later sculptures were carved out of earlier ones, and some are only half-completed. This suggests that perhaps rival cities or tribes came to control the countryside, making the acquisition of new stone difficult. Another possible explanation is that if the population somehow declined, there would be insufficient manpower to quarry and transport new material. The era around 900 B.C. is also historically linked to some climatic changes, which could well have adversely affected San Lorenzo. As a relatively primitive, developing culture, the people of San Lorenzo subsisted on a handful of core crops, hunting, and fishing. A sudden change in climate could affect these crops, as well as the nearby wildlife. San Lorenzo, while not a spectacular place for visitors like Chichà ©n Itz or Palenque, is nevertheless an extremely important historical city and archaeological site. The Olmec is the parent culture of all of those which came later in Mesoamerica, including the Maya and Aztecs. As such, any insight gained from the earliest major city is of inestimable cultural and historical value. It is unfortunate that the city has been raided by looters and many priceless artifacts have been lost or rendered valueless by being removed from their place of origin. It is possible to visit the historical site, although many of the sculptures are currently found elsewhere, such as the Mexican National Museum of Anthropology and the Xalapa Anthropology Museum. Sources Coe, Michael D. Mexico: From the Olmecs to the Aztecs. Ancient Peoples and Places, Rex Koontz, 7th Edition, Thames Hudson, June 14, 2013. Cyphers, Ann. San Lorenzo, Veracruz. Arqueologà ­a Mexicana, No. 87, 2019. Diehl, Richard. The Olmecs: Americas First Civilization. Ancient Peoples Places, Hardcover, Thames Hudson, December 31, 2004.

Discuss the ways of platelets storage Essay Example | Topics and Well Written Essays - 500 words

Discuss the ways of platelets storage - Essay Example The first and foremost step after the collection for separating platelets is to screen the blood for bacteria as well for other diseases which include hepatitis B, hepatitis C and HIV (Kaufman 2006). Also another step before the separation of platelets is to actually make all the preparations before the blood is drawn from the donor. This includes making all the necessary preparations so that the withdrawal procedure is made sterile from the point of blood drawing till the point of infusion to the patient (Tullis et al 1959). The blood is drawn from the patient in a container which contains ACD solution added to it. It is also ensured that blood is drawn in a clean method with no requirement for giving a second puncture as this is an important protocol of platelet collection that blood should be drawn from one puncture. This blood is circulated through a tube of plastic which is maintained at a temperature of 4 degrees centigrade. The blood flows in this chamber and the residue that is left flows into a separate pool and this residue is basically the plasma which is devoid of red and white blood cells and contain only platelets. This platelet containing plasma is then passed through another chamber such that 10 ml of the fluid flows in every one minute. The platelets flow into a collector where they are maintained at 250 x gravity.

Professional Role Transition Assignment Example | Topics and Well Written Essays - 750 words

Professional Role Transition - Assignment Example Data sources that contained information related to the topic were also used during the research process. The studies presented evidences such as quoting those who were interviewed during the study. This showed that students were given equal opportunity to answer the questions directed at them. The studies also took a while to be completed. A slow research ensures that the researcher gains all the information required. Things tend to change with time and by conducting a research study within a long time, the researcher gets to record every change that may take place and also predict how the study will be in future. What I know about the journal is that it intends to understand the challenges that students face when transitioning into BN from LPN. The journal identifies all the challenges and helps to explain methods that can be used to curb them. The journals target LPN students who may be interested in studying BN. The study will help them understand the challenges they are bound to face. The journals also target other scholars that focus on studying the topic. These journals will give them a point of reference for their study. Educators in the institutions that the students enroll for BN are also a target audience for the study because they will have the knowledge of how to help the students adapt to the transition. Because of the challenges faced when transitioning from LPN to BN, students opt to turn to their mentors at their places of work to guide them. These mentors are in a great position to help them put into practice what they have studied. The journals have also helped explain why the LPNs choose to expand their knowledge. One of the reasons given by the participants is that at the work place, they are considered less of nursing practitioners and their opinions are not valued. Some of them had to endure being called Little Pretend Nurses instead of their real titles. For the students who have

Streak Plate And Viable Cell Count

Streak Plate And Viable Cell Count Aim and introduction should display insight into what the streak plate and viable cell count method are employed to achieve. They should also introduce MacConkey agar and how its selective and differential properties allow the characteristics of the test organism to be determined. Escherichia coli (E.coli) The aim of this experiment is to allow a certain bacterium to divide and multiply enabling us to view the bacteria in a single cell structure. E.coli is one bacterium that is good for such an experiment. E.coli can be said to be both bad and harmless, some E.coli bacteria have are highly toxic and can harm humans and animals. However, the majority of E.coli strains are relatively harmless with low toxicity. These harmless strains of E.coli are found naturally occurring in the human body, especially in areas such as the human intestines. Some E.coli can even benefit their hosts; they do this by producing specific vitamins. It is for reasons like the ones mentioned why E.coli is an appropriate bacteria to use for this experiment. Another reason is that E.coli bacterial cells have an average bacterial size of 2um; this can be seen under a light microscope. Other bacteria however may be even smaller and may require a larger microscope for viewing or even an electron microscope. Also the incubation period for E.coli to multiply and grow rapidly isnt very long and temperatures arent too high or too low. E.coli can be incubated overnight at 37oC and then stored at 4oC until its requirement. The technique use to manipulate and isolate the E. coli bacteria is known as the streak plate procedure. The technique was developed to allow bacteria to multiply and produce many colonies, during the incubation period, depending on the amount of bacteria present. Each colony will contain millions of bacteria cells derived from a single parent cell. (Talk in more detail about this procedure). We will be using this technique to allow the E. coli to multiply and divide splitting itself into colonies. The viable cell count, also known as viability count, is a method used to determine the number of living cells within a suspension, in this case E. coli. To obtain an understanding of how much E. coli cells are present in a sample this method must be put into action. (Expand) The MacConkey agar is specifically designed to allow gram negative bacteria to grow; its a recipe of many substances such as bile salts, sodium chloride proteose and many more. One of the properties of the MacConkey agar is its selective isolation and identification of bacteria; it is a medium that allows us to distinguish gram-negative bacteria. E. coli is a rod shaped gram negative bacteria, so using the MacConkey agar plate to multiply it would be appropriate, the agar will also cause the E. coli to change colour from pink to red, and this is an indication of gram negative bacteria present. A Nutrient agar is a growth medium used for the cultivation of bacteria, this specific agar remains solid even at high temperatures. The gram-stain technique was developed for viewing cells clearly under a microscope and to enable us to establish their structures. It is a very simple procedure of just adding 4 different substances accordingly, however one of these substances is toxic to humans therefore the procedure must be carried out in a fume hood. The gram stain method was introduced by It is important for scientists and medics to know the structure and function and identity of bacteria and viruses, it is for reasons like this why such experiments are carried out. Without such procedures so many bacteria and viruses wouldnt be known and could spread and become out of control. Methods: Explain why each procedure was done highlight key points state any deviation from protocol document any errors or difficulties you had with the technique Discuss the importance of aseptic technique and what steps could be taken to prevent contamination during manipulation of bacteria All methods were doing under aseptic conditions; the reason for this is to prevent contamination of the bacteria during its manipulation. Many errors could arise if aseptic conditions arent used, eventually resulting is wrote results. Streaking bacteria on MacConkey agar method: Prior to the experiment, an E. coli sample was made and given to during the practical. Risk assessment: Materials used: 10ml liquid culture of E. coli the bacteria sample to be use in this practical Sterile plastic loops used for transferring E.coli bacteria from one place to another uncontaminated. MacConkey agar plate used to allow E.coli bacteria to grow as it provides energy recourses and support Sharps bin for loops etc. these are used to keep the laboratory area as uncontaminated as possible and to make sure bacteria doesnt spread Marker pens and labels to label the plate Step 1: using a sterile plastic loop I touched a given sample of E. coli and streaked an inoculum onto a MacConkey agar plate in a specific pattern (see..). This plastic loop is then disposed of into the sharps bin. Step 2: using another sterile plastic loop, I created a run of parallel lines from the edge of the initial streaks Step 3: step 2 was repeated 2 more times with a new sterile loop used. Step 4: a final streak was made, creating a simple streak from the previous streaks into the centre of the plate. The picture below illustrates this. The MacConkey plate was then given to the technicians to incubate. It was important to dispose- on the plastic looks by placing them into the sharps bin because they are contaminated and if they touch any other surface it can lead to the spread of bacteria resulting in major contamination. Throughout this procedure plastic gloves and a lab-coat were worn, also to prevent contamination. Viable cell counts: Risk assessment Materials used: P1000 and P100 pipettes and tips used to transfer certain amounts of PBS etc. Three Nutrient agar plates 10ml sterile PBS buffer, used to maintain the pH Sterile plastic spreaders to spread the E.coli on the Nutrient agar plates Eight sterile bijoux bottles for dilutions To start the dilution, using a pipette I transferred 900 ul of diluents (PBS) in eight different sterile bijoux bottles. The PBS (phosphate buffered solution) solution is a commonly used buffer to maintain a pH; it is used in this practical because of its ability to aid biological research. After the PBS was placed into the labelled bottles, using a new sterile tip for the pipette I transferred 0.1ml of E.coli liquid culture sample (neat) into the first bottle (10-1). For the dilution to continue a new pipette tip was placed and 0.1ml of the 10-1 diluted E.coli was transferred to the 10-2 bottle, this process continued up till bottle 10-8. By doing so the E.coli will become more and more dilute within the different solutions, because less E.coli is being added each time. 10-5, 10-6 and 10-7 samples were then spread onto three different Nutrient agar plates using different sterile plastic spreaders so contamination wouldnt occur. This was done by placing 0.1ml of each dilution onto th e centre of the agar plate and then spreading it over with a sterile spreader. The agar plates were labelled and given to the technician for incubation. Gram Stain of bacteria from an isolated colony Risk assessment In order to stain the bacteria I selected an appropriate colony to stain, the colony must appear to be uncontaminated and its appearance must obviously look grown.. After this procedure is complete, the bacterial cells will be visible under a microscope. Materials needed: Bunsen burner used to heat-fix bacteria onto microscope slide Saline (PBS) emulsifier Light microscope to view bacterial cells Lens tissue to clean the lens Immersion oil for light microscope lens to allow better view at 100x magnification Stains for Gram stain method Before the bacteria can be modified to be viewed under the microscope clearly, the microscope glass slide must be cleaned to prevent contamination. After doing so a drop of sterile saline was placed onto the centre of the slide, the saline drop was placed because it can emulsify any bacterial colony that will be placed on top. To move some of the bacteria off the agar plate onto the slide, a sterile loop was used I touched the bacterial colony on the agar plate with the top of the loop and then spread the bacteria into the saline drop making it thin. Due to the moisture of the liquid I let the slide dry then used a Bunsen-burner to heat-fix the bacteria onto the slide by passing it through a few times then allowing it to cool. Heat-fixing was done so that during the staining the bacteria or wouldnt move or fall off. Once that was complete the slide was moved to a laboratory fume hood where the staining can take place, the follow 4 stage method was used: at first the bacteria sample on the slide was soaked in crystal violet for 30 seconds, after so it was rinsed with distilled water and drained. The second part is to soak the bacteria with gram iodine (mordant) for another 30 seconds then rinse with distilled water and drain it. Gram iodine is a toxic substance; it is for this particular reason why this part of the practical was carried out in a laboratory fume hood. Acetone decolouriser was then added for 10 second and the bacteria was again rinsed with distilled water and drained. The final part is to add Safranin, a counter stain, to the bacteria. It was placed on the bacteria for 30seconds and then the bacteria was further rinsed with distilled water, drained, blotted and allowed to dry. Substance Duration Further action Crystal violet (primary stain) 30 seconds Rinse with water drain Gram Iodine (Mordant) 30 seconds Rinse with water drain Acetone/alcohol (Decolouriser) 5-10 seconds Rinse with water drain Safranin (Counter stain) 30 seconds Rinse with water, drain, blot dry Stain was carried out in a laboratory fume hood due to the toxic gram iodine substance used. The transparent plastic shield of the fume hood was lowered so that only my hands were inside dealing with the chemical and biological substances. Gloves were worn during this procedure so that no stain would come into contact with the skin. When the slide was rinsed with water, it was rinsed gently with distilled water so that the bacteria are not shifted. After the staining was completed the sample can now be viewed under a light microscope and compared to other bacterial samples. The slide is placed on the stage with a drop of oil for immersion, the microscope is focused on 100x and the bacteria is viewed. Results: Should describe your findings in : prose/text, diagrams, tables and graphs which includes a description of growth characteristics and how successful your aseptic technique was MacConkey agar plate results: During the experiment there were no results to be noted as it was too early for anything to occur. After the agar plate containing the E.coli was incubated at 37oC and then stored at 4oC, its appearance was as expected. Colonies were separated, and as the streaks moved on less E.coli was present. The colonies were well distinctive and were round in their shape. The sample initially given was simply liquid, the result showed significant growth of this E.coli liquid into 3D structures. This indicates the growth of the bacteria in a fine way; the 3D structures appeared in a yellowing solid colour. Because the practical was conducted in aseptic techniques no contamination occurred. Aseptic techniques were successful in allowing me to produce accurate results. Viable cell counts My results: The colonies that appeared on the nutrient plate had a badge colour; visually they all appeared relatively same sized and volume. 10-5 10-6 10-7 10-8 TMTC 46 1 For the 10-7 the calculation for the number of bacteria in 1ml of the original culture is: (1107/0.1) x (X/1) [cross multiply] 0.1X = 1 x(1107) [divide by 0.1] Therefore X = 1.0108 For the 10-6 the calculation for the number of bacteria in 1ml of the original culture is: (46106/0.1) x (X/1) [cross multiply] 0.1X = 1 x(46106) [divide by 0.1] Therefore X = 4.6108 The number of bacteria present in 1ml of the 10-5 culture cannot be calculated as there was no value noted (TMTC). X= number of bacteria. The number of bacteria present in 1ml of 10-6 dilution is 4.6108 and in the 10-7 dilution culture is 1.0108. Class results: Pair number 10-5 10-6 10-7 10-8 1 TMTC 46 1 2 95 9 0 3 TMTC 52 9 4 TMTC 23 1 5 34 2 6 84 11 4 7 TMTC 24 18 8 2 2 26 9 102 6 0 10 19 3 3 11 TMTC 140 15 12 TMTC 57 9 13 61 12 0 14 8 6 1 15 195 51 4 16 55 27 3 17 TMTC 94 11 18 TMTC TMTC TMTC 19 TMTC TMTC TMTC 20 TMTC 113 18 Total 266 985 177 28 Average 66.5 57.9 9.8 7 Should describe your findings in : prose/text, diagrams, tables and graphs which includes a description of growth characteristics and how successful your aseptic technique was To determine the amount of bacteria within a culture a simple calculation must be done using my personal results for this experiment. There was no value for the 10-5 so this cannot be done. The result for 10-6 was 46, 46 x 10 = 460ml. To estimate the amount of E.coli present this is further multiplied by 106, therefore 460 x 106 = For the 10-7 result Æ’Â   7 x 10 = 10 Æ’Â   10 x 107 = However, I have selected some reasonable results from the table to calculate an average. Gram-stain of bacteria from an isolated colony: (view method number 3) Gram stains help us distinguish between microbial organisms, for example gram negative bacteria and gram positive bacteria. This method was developed to know the identity of bacteria present. (See procedure for The Gram Stain in the methods section). During step 5 of the Gram Strain Method above the following results were made when applying the four different substances: Substance Colour after stain Crystal violet (primary stain) Purple Gram Iodine (Mordant) Purple Acetone/alcohol (Decolouriser) Transparent (dye was washed off) Safranin (Counter stain) Reddish-pink The appearance of the E.coli bacteria under a microscope with 100x magnification was quite clear; it had a rod-like structure with a reddish-pink colour. The rods were all more or less the same size, however some were packed together and others were on their own. Discussion: Were the results the expected? Did the methods adopted achieve their aim? How the experiments could be improved. Include background information, critical evaluation of results Throughout all the experiments and procedures a lab-coat and gloves were worn to avoid skin contact with bacteria and harmful substances. Overall the aims were accomplished and the results were as predicted. MacConkey agar The colonies were expected to be in such a form, indicating that it was E.coli present and that it has rapidly multiplied into individual colonies. This further suggests that when E.coli is present under conditions where it could multiply, it multiplies by forming a round colony and expanding from there. However, some of the colonies were stuck together making it difficult to count the number of them present. What this means is that the growth of the bacteria was a success and the method adopted was accurate. The reason why some colonies were packed together may be the result of pressing too hard on the agar while streaking, with more streaking practice more accurate results can be obtained with colonies being on their own. The methods adopted for this practical achieved what was aimed for. After the incubation of the MacConkey agar plate the plate was stored for a week at a temperature of 4oC, this may have changed the appearance in colour and in shape of the formed colonies. Contam ination of the agar plate may have even occurred. An improvement to the experiment is to note down results straight after incubation is finished. Gram Stain results After analysing the microscope slide which contains the Gram Stained E.coli under the microscope its features were obvious. There were many average sized rods with a reddish-pink colour, some of these rods were packed together whist others were separated. Comparing this with another prepared sample of B.subtilis, the B.subtilis was a purple colour and has a longer and curved shape, like fine threads. However some again were packed together and others separated. The purple colour of the B.subtilis indicates that it is gram positive, and the pink colour of E.coli indicates its gram negative. When the Gram Stain method was applied to the B.subtilis it obviously stayed purple though out, with E.coli it will decolourise once the decolouriser is added. The gram stain method is highly effective and efficient when dealing with different bacteria; it helps identify them to a great extent. B.subtilis remains purple throughout the Gram Stain procedure, this itself can be an indication that it is a Gram positive bacteria. Bacterial cells have different types of cell walls, the gram negative and gram positive terms describe the nature of their structural differences. One of the important differences is that Gram positive bacteria have no outer membrane whereas Gram negative bacteria do, the purpose of this outer layer is to cover the peptidoglycan layer. When staining occurs the outer membrane of a gram positive bacterial cell wall becomes permanently stained as the strain can easily penetrate the thick peptidoglycan layer, so that if a decolouriser or distilled water is added the colour will remain purple. In the case of the gram negative bacterial cell wall the stain gets attached to the far outer membrane layer (lipopolysaccharide and protein), this layer decreases the penetration depth of the strain on the peptidoglycan, so the stain can be decolourised or removed. The diagrams below illustrate this. Gram positive: Æ’Â   Æ’Â   Æ’Â   Primary stain Mordant Decolourisation Counter-stain Note: colour remains the same throughout addition. Gram negative: Primary stain Mordant Decolourisation Counter-stain Note: colour changes Æ’Â   Æ’Â   Æ’Â   The aim of the Gram Stain method was confirm that the bacteria that was initially being dealt with was E.coli, after tests and results it confirmed that it was so the results were as expected and predicted. The methods used for this procedure were successful at achieving good results, however some can be altered. For example, the E.coli used for this experiment was used from experiment number one, not that that is a problem but when the E.coli was incubated over night and it had successfully multiplied it was stored at 4oC for quite a while (this experiment was carried out 1 week after the first one). This possible may have altered the activity of the E.coli and also its appearance. Many resources state that gram negative bacteria should have a pink colour after the counter-stain has been added and rinsed off. In this case the E.coli bacteria in this experiment had quite a dark pink colour which was really close to the colour red, this appearance of colour was visual both with the naked eye and under the microscope as individual bacterial cells. Viable cell counts As I predicted, the more dilute (10-8) solution will have less E.coli bacteria growing on its surface. As there were 20 different pairs doing the practical, and the dilutions were all done 20 times by different people, there is plenty room for error from contamination of inaccurate measurements. The 10-5 agar plates had many E.coli bacterial colonies growing on it, according to the results there was far too many bacteria that it was too many to count (TMTC). Gradually as the dilution increased the bacteria became less, 10-6 dilution had numbers ranging from 6-140. Obviously with such great difference within what is meant to be the same dilution there was some error/contamination present. The most obvious ones which had error are pair numbers 18 and 19 there was TMTC throughout (10-6, 10-7 and 10-8). What would be expected is that fewer bacteria should be present in the 10-8.

Personalities of The Cold War Essay

Who caused the cold war? Focus; the role of each personality in contributing to the cold warTime frame: 1945 to 1952Cold war- period of intense tension and mistrust, leading to competition and confrontations. Stand: both Stalin and Truman contributed to the cold war. The key personalities that contributed to the cold war are namely Stalin and Truman,both in office in US and USSR respectively. both were responsible as their personalities and level of experiences contributed to their policies made, which heightened tensions and thus caused the cold war. Personality traits like Stalin’s paranoia gave rise to his expansionist policy, which hardline Truman viewed as aggression and tried to counter it in the Truman Doctrine and Marshall plan. Truman’s low level of experience in dealing with Stalin also increased tensions and led to the cold war. Stalin was aggressive and protective of the USSR as he was a true hardline communist and believed that Russia had to stay strongly communist. However, the USSR was invaded thrice in no more than a century and also suffered civil war and intervention from anti-communist forces from 1918 to 1920, when communism as an ideology was at infancy. These anti-communist forces comprised of the West when they helped the Whites during the Bolshevik Revolution. From this, Stalin believed that the West wanted to destroy communism before communism became stronger. His mistrust grew as he became paranoid and thought of the West as a potential security threat this was because This prompted his embarking on salami tactics ( an expansionist policy) so that Communism would remain strong in Eastern Europe. Gradually the Russians began to systematically interfere in the countries in Eastern Europe to set up pro-communist governments, in countries like Poland, Hungary, Bulgaria, Albania and Romania. Stalin felt that his actions were justified for the defense of communism, and that salami tactics were absolutely necessary. He did not realize that his actions had frightened the West. What he failed to consider was that the west was not interested in destroying communism but was looking more towards post war cooperation. The  west showed this through the decisions made at the post-war conferences when Russia was allowed to take reparations from Germany and it was allowed to benefit from the loans from the west, known as the Lend Lease. His paranoia in embarking on the expansionist policy ( comprising Salami tactics) when juxtaposed with Truman’s hardline views, only served to heighten suspicion and tensions, leading to the Cold war. Similarly, Truman became suspicious of the USSR’s intent towards eastern Europe. He was a hardline president who stood firmly against Communism, and was intolerant of the needs of the USSR . Truman saw Stalin’s actions as a breach of the Declaration of Eastern Europe where Stalin had promised to allow countries like Poland free elections but failed to do so, instead carrying out the reverse and forcing communist governments in these countries through rigged electionsTruman, with his hardline view that communism was bad, viewed Stalin’s moves as being remarkably similar to Hitler’s salami tactics, and that the USSR was embarking on aggression. In addition he felt that if he did not stop Stalin, Stalin would think that Eastern Europe was his for the taking, and capitalism would perish. This in turn led to the formation of the Truman Doctrine and Marshall plan which would serve to increase tensions (elaborated below)Secondly, the Truman Doctrine and the Marsh all Plan. The West formulated the Truman Doctrine and the Marshall plan to contain communism in Greece and Turkey. Communists were trying to overthrow the monarchy but british troops who restored the monarchy in the past were feeling the strain of supporting it against the communists. The british prime minister appealed to the USA and Truman announced that it would † support free peoples who are resisting subjugation by armed minorities or by outside pressure’ and Greece received massive amounts of aid and the communists were defeated. The funds of the Truman doctrine were obtained through Congress where Truman portrayed the situation in Greece and Turkey as part of the global communist threat. Truman’s low level of experience prevented him from realizing that Stalin would see the Truman doctrine as an attempt to subvert them. In other words, he did not deal with stalin before, and did not see that his portrayal was excessively confrontational and would  serve to heighten Stalin’s paranoia and escalate his mistrust towards the West. Similarly, the Marshall plan was formulated to facilitate economic recovery in Eastern Europe. By September, 16 nations had drawn up a joint plan for using American aid and in total over 13000 million dollars of Marshall Aid was given to western European countries. However, the west did not consider the impact that the Marshall plan would have on how the USSR viewed them. Truman’s low level of experience with dealing with the Stalin prevented him from seeing that the Stalin was sensitive about USSR’s economic status (Stalin refused to ratify the the Bretton Woods agreement so that the West would not realize how economically weak the USSR was in 1945. The Bretton woods agreement was a system to acquire international currency stabilization which required foreign access to sensitive economic data. Stalin viewed the policy as ‘dollar imperialism† and as a blatant American device for gaining control of western Europe, and made all Russian states reject the offer. The Truman doctrine and the Marshall plan would eventually prompt Stalin( along with his paranoia) to come up with the Cominform and the Comecon which served to unify all Eastern European satellite states. Truman’s low level of experience in coming up with the Truman Doctrine and the Marshall plan, when juxtaposed with Stalin’s paranoia, fueled further consolidation of power by Stalin, which escalated tensions on both the USA and the USSR and caused the Cold war. Biblography: Richard Crockett, the fifty years war: The United States and the Soviet Union in World Politics, 1945-1991Gaddis, John Lewis. We now know: Rethinking Cold war History. US: Oxford University Press.

National Response Plan Howard L. Hayes Saint Leo University

National Response Plan Howard L. Hayes Saint Leo University Abstract Why is it necessary for our country to have an effective emergency plan in place? Why does it matter how the country responds to emergencies when every part of our country, including cities and states, face emergency situations on a daily basis all over? Some are bad to worse, and some horrific to deadly. Well, this paper explains the importance of the National Response Plan and why it is necessary for our country to have it in place. It explores several articles that have been published to show the results on the effectiveness and response of the National Response Plan. The National Response Framework, previously called The National Response Plan, is a plan that the United States put in effect to organize the way the country and the community handles attacks, disasters, as well as all events looked at as an emergency. The Response Plan relays and describes all the information in order to avoid catastrophic damages and to protect and save lives. The plan focuses on how the country as a whole can work together to prepare for damaging events. Morover, the plan doesn t always seem to be effective as it is expected to be. Issues arose at the time it is most needed, which causes necessary changes to take effect immediately. Sometimes the damage is already done and that could be due to economic collapse, being unprepared, and failure to respond appropriately. The National Response Framework replaced the NationalShow MoreRelatedNational Response Pl An Effective Emergency Plan1321 Words   |  6 Pages National Response Plan Howard L. Hayes Saint Leo University Abstract Why is it necessary for our country to have an effective emergency plan in place? Why does it matter how the country responds to emergencies when every part of our country, including cities and states, face emergency situations on a daily basis all over? Some are bad to worse, and some horrific to deadly. Well, this paper explains the importance of the National Response Plan and why it is necessary for our countryRead MoreManagement Course: Mba−10 General Management215330 Words   |  862 Pagestwenty-first century’s economic, social, and political churning, how will these driving factors be influenced by the brutally competitive global economy in which organizations do not have any particular geographic identity or travel under any particular national passport? What will be the effect of the rapid gyrations in markets that emphasize the difficulties that accounting practices face in determining true performance costs and that forecasting programs confront in establishi ng the economic determinants

The Effect Of Cell Phones On Teenager s Lives - 843 Words

The Effect of Cell Phones in Teenager’s Lives iPhone 5, 5s, 5c, 6, 6XL, Droid, Blackberry. The list of different cell phones that are in the possession of the teenage population across the world, is endless. The use of a cell phone especially in the teenage years, is almost like an addiction. This is mostly because of the endless possibilities having to do with a cell phone. Today cell phones are basically mobile computers at your fingertips. Within a second the Internet can be searched for anything, at any time or any place. Games can be played any where, in conjunction to any app. Pictures can be taken and sent out, being deleted instantly all with the use of Snapchat. Social media apps like Twitter, Facebook, and Instagram, allow interaction with friends to see what they’re up to at that very moment. With all of these features being available to a teenager, why wouldn’t they never want to stop using it? The effects of cell phones in a teenager’s life are grades dropping and texting and driving rate s increasing. With the increase of teenagers having cell phones comes the use of them in school, which leads to a decrease in grades. Texting is one of the main purposes of a cell phone in the teenage years. With texting comes the distraction to pay attention; looking at the phone, rather then the lesson in class. Studies show that â€Å"students are texting frequently during class time, and that interferes with their ability to pay attention and learn† (â€Å"Texting inShow MoreRelatedA Life Threatening Habit : Using Drugs And Alcohol1665 Words   |  7 PagesUsing a cell phone may be equivalent to using drugs and alcohol. Addiction to cell phones is a worldwide problem. There are many negative effects that teenagers do not know and that will cause harm in long term usage. For some, using their cell phone is a way out of reality and an escape from the outer lives. 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