Nco Disrespect Towarsds Essay Sample free essay sample

Disrespecting An NCO Essay Today this essay will be about disrespecting a Non Commission Officer or ( NCO ) . There is many different ways and form’s of disrespecting an NCO. One manner that you could disrespect an NCO is the manner you talk. move. facial motions. how your voice is. your organic structure linguistic communication. and most of all how you present yourself towards an NCO. Lets start off with the manner you talk. The manner you talk can be a large factor in how your SGT can take it. So the best manner to avoid any confrontation between you and your SGT is to ever hold to what there stating. When they tell you something you do it. Its yes SGT Roger SGT. Its non good SGT. possibly SGT. or No SGT. You ever have to be respectful towards Your SGT because in the military its all about the superior system. We will write a custom essay sample on Nco Disrespect Towarsds Essay Sample or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page You may non esteem the individual but you have to esteem the rank. So you ever have to be respectfull when there speaking to you. Now the following thing that’s a large factor on being respectful towards a NCO is on how you move. When a NCO negotiations to you. You ever have to stand at the place of parade remainder. You have to stand up consecutive and with shoulder back. cervix back and when there speaking to you you have to look them directly in the oculus. Now the following thing to make to esteem NCO is your facial motions. When your NCO’S are speaking to you have to look them directly in the oculus without any facial motions. you cant cock your oculus brows you cant turn over your eyes. you have to reasonably much have no facial looks. You have to look them directly faced non laughting nil. And now one of the last things about esteeming NCO’S is the manner your voice is towards the NCO. When you talk to person like I said you have to be like yes sgt. no sgt. roger sgt. and when there speaking to you. You cant have that toan in your voice where it sounds like your seeking to revenge. you cant have the voice like your seeking to be better so your NCO. You have to cognize that you are non above he or she. Like me personally im merely a private so I don’t have a say so in how things work. I cant act like im better so the certain individual. Or it will consequence my hereafter and ruein me for the remainder of my life. So that’s why I try to be the most respectful I can be. But some times it is so difficult to make because I have that revenging image in me from when I was turning up I feel like I don’t need to be taken attention of and I don’t like people giving me counsel because I feel I can make it on my ain and if I don’t do it a certain manner I feel like I get in problem for no ground. I mean if I can make it a manner that’s works best for me and make the right manner I feel like it will work out. Now I believe this is the last thing about disrespecting NCO is how you present yourself towards a NCO.

A Memorable Event in My Life Essay Example

A Memorable Event in My Life Essay Example A Memorable Event in My Life Paper A Memorable Event in My Life Paper My Life Being stuck In one of the most remote places In Cambodia does not seem like part of a vacation plan for anyone. In our lives, there will always be times when things do not go as the way you planed. Due to not preparing for any emergencies, our family learned a very important lesson in life. I was 13 when this incident happened. My family and I were on holiday in Sandhog province when we were left praying for help as our car got caught in the wet and muddy soil on the border of Sandhog and Henna province. We had been driving for six hours when we reached Luminous. The stone that made up the road became dirt, buildings turned Into trees and cars turned Into cows. As we moved on, our surrounding became more and more remote. Soon there was no sight of any human being, only dirt roads stretched out like veins running through our bodies. The wind of the coming storm began to pick up speed. Trees swayed from side to side as the gloaming sky rumbled and the lighting flashed. I tried to control my fear but the sound of the storm seems to suppress my ability to control my emotions. Then the unthinking happened. Two of our back wheels were sucked by the muddy soil; It could no long propel us forward. We were stuck. Father stepped hard on the gas pedal over and over again but it was in vain. Trapping in the middle of nowhere turned my fear into tears. What happen if there were bandits? What happened if there was a landslide? I could not help but thought of all the possible ways of how I will die. This doesnt seem good, wave got to hurry. Fathers words seem to have woken me up from a dream. I was no longer crying. The fear of dying gave me strength. I got out of the car and with all of my strength, I pushed. However my effort yields no result. Then an Idea struck me. I went to the back of the car and grabbed the two paintings that I have bought in Kingdom and placed it under the back wheels. Again, we pushed. As I hoped and prayed, the car went over the painting and we were freed. Smiles followed by relieve were on everyones face. As e continued on with our Journey, the rain did not stop and the chances of us trapping In the muddy soil were still there but this time I was ready. I would not sit and Walt for father to save me. My fear had turned Into strength. For the rest of the journey we trapped in the muddy soil for three more times but we managed to overcome the obstacle as one. This incident made me realized that no matter what difficulties we faced, as long as we have the perseverance, determination and the willingness to work as one, we will be able to overcome it.

The Historic Olmec City of San Lorenzo

The Historic Olmec City of San Lorenzo The Olmec culture thrived along Mexico’s Gulf coast from roughly 1200 B.C. to 400 B.C. One of the most important archaeological sites associated with this culture is known as San Lorenzo. Once, there was a great city there. Its original name has been lost to time. Considered by some archaeologists to be the first true Mesoamerican city, San Lorenzo was a very important center of Olmec commerce, religion, and political power during its heyday. Location San Lorenzo is located in Veracruz State, about 38 miles (60km) from the Gulf of Mexico. The Olmecs could not have selected a better site to build their first great city. The site was originally a large island in the middle of the Coatzacoalcos River, although the course of the river has since changed and now only flows past one side of the site. The island featured a central ridge, high enough to escape any flooding. The floodplains along the river were very fertile. The location is close to sources of stone which were used for making sculptures and buildings. Between the river on either side and the high central ridge, the site was easily defended from enemy attack. Occupation of San Lorenzo San Lorenzo was first occupied around 1500 B.C., making it one of the oldest sites in the Americas. It was home to three early settlements, referred to as the Ojochà ­ (1500-1350 B.C.), the Bajà ­o (1350-1250 B.C.), and the Chichrras (1250-1150 B.C.). These three cultures are considered pre-Olmec and are largely identified by pottery types. The Chicharrs period begins to show characteristics later identified as Olmec. The city reached its peak in the period from 1150 to 900 B.C. before falling into decline. This is referred to as the San Lorenzo era. There may have been some 13,000 inhabitants at San Lorenzo during the height of its power (Cyphers). The city then went into decline and passed into the Nacaste period from 900 to 700 B.C. The Nacaste did not have the skills of their forebears and added little in the way of art and culture. The site was abandoned for some years before the Palangana era (600-400 B.C.). These later inhabitants contributed some small mounds and a ball cou rt. The site was then abandoned for over a thousand years before it was re-occupied during the Late Classic era of Mesoamerican civilization, but the city never regained its former glory. The Archaeological Site San Lorenzo is a sprawling site which includes not only the one-time metropolis of San Lorenzo but several smaller towns and agricultural settlements that were controlled by the city. There were important secondary settlements at Loma del Zapote, where the river forked to the south of the city, and El Remolino, where the waters re-converged to the north. The most important section of the site is on the ridge, where the nobility and priest classes lived. The western side of the ridge is known as the â€Å"royal compound,† as it was home to the ruling class. This area has yielded a treasure trove of artifacts, particularly sculptures. The ruins of an important structure, the â€Å"red palace,† are also found there. Other highlights include an aqueduct, interesting monuments scattered around the site, and several artificial pits known as â€Å"lagunas,† the purpose of which is still unclear. Stonework Very little of Olmec culture has survived to the present day. The climate of the steamy lowlands where they lived has destroyed any books, burial sites, and items of cloth or wood. The most important remnants of the Olmec culture are therefore architecture and sculpture. Fortunately for posterity, the Olmec were talented stonemasons. They were capable of transporting large sculptures and blocks of stone for masonry for distances of up to 60 kilometers (37 miles). The stones were probably floated part of the way on sturdy rafts. The aqueduct at San Lorenzo is a masterpiece of practical engineering. Hundreds of similarly-carved basalt troughs and covers weighing many tons were laid out in such a way as to promote the flow of water to its destination, which was a duck-shaped cistern designated Monument 9 by archaeologists. Sculpture The Olmec were great artists and the most remarkable feature of San Lorenzo is doubtless the several dozen sculptures that have been discovered at the site and nearby secondary sites like Loma del Zapote. The Olmec were famous for their detailed sculptures of colossal heads. Ten of these heads have been found at San Lorenzo. The largest of them is nearly ten feet tall. These massive stone heads are believed to depict rulers. At nearby Loma del Zapote, two finely sculpted, nearly identical twins face two jaguars. There are also several massive stone thrones at the site. All in all, dozens of sculptures have been found in and around San Lorenzo. Some of the statues were carved out of earlier works. Archaeologists believe that the statues were used as elements in scenes with religious or political meaning. The pieces would be laboriously moved around to create different scenes. Politics San Lorenzo was a powerful political center. As one of the first Mesoamerican cities - if not the first - it did not have true contemporary rivals and ruled over a large area. In the immediate environs, archaeologists have discovered many small settlements and dwellings, mostly located on hilltops. The smaller settlements were likely ruled by members or appointments of the royal family. Smaller sculptures have been found at these peripheral settlements, suggesting that they were sent there from San Lorenzo as a form of cultural or religious control. These smaller sites were used in the production of food and other resources and were of strategic use militarily. The royal family ruled this mini-empire from the heights of San Lorenzo. Decline and Importance In spite of its promising start, San Lorenzo fell into steep decline and by 900 B. C. was a shadow of its former self. The city would be abandoned a few generations later. Archaeologists do not really know why San Lorenzos glory faded so soon after its classic era. There are a few clues, however. Many of the later sculptures were carved out of earlier ones, and some are only half-completed. This suggests that perhaps rival cities or tribes came to control the countryside, making the acquisition of new stone difficult. Another possible explanation is that if the population somehow declined, there would be insufficient manpower to quarry and transport new material. The era around 900 B.C. is also historically linked to some climatic changes, which could well have adversely affected San Lorenzo. As a relatively primitive, developing culture, the people of San Lorenzo subsisted on a handful of core crops, hunting, and fishing. A sudden change in climate could affect these crops, as well as the nearby wildlife. San Lorenzo, while not a spectacular place for visitors like Chichà ©n Itz or Palenque, is nevertheless an extremely important historical city and archaeological site. The Olmec is the parent culture of all of those which came later in Mesoamerica, including the Maya and Aztecs. As such, any insight gained from the earliest major city is of inestimable cultural and historical value. It is unfortunate that the city has been raided by looters and many priceless artifacts have been lost or rendered valueless by being removed from their place of origin. It is possible to visit the historical site, although many of the sculptures are currently found elsewhere, such as the Mexican National Museum of Anthropology and the Xalapa Anthropology Museum. Sources Coe, Michael D. Mexico: From the Olmecs to the Aztecs. Ancient Peoples and Places, Rex Koontz, 7th Edition, Thames Hudson, June 14, 2013. Cyphers, Ann. San Lorenzo, Veracruz. Arqueologà ­a Mexicana, No. 87, 2019. Diehl, Richard. The Olmecs: Americas First Civilization. Ancient Peoples Places, Hardcover, Thames Hudson, December 31, 2004.

Discuss the ways of platelets storage Essay Example | Topics and Well Written Essays - 500 words

Discuss the ways of platelets storage - Essay Example The first and foremost step after the collection for separating platelets is to screen the blood for bacteria as well for other diseases which include hepatitis B, hepatitis C and HIV (Kaufman 2006). Also another step before the separation of platelets is to actually make all the preparations before the blood is drawn from the donor. This includes making all the necessary preparations so that the withdrawal procedure is made sterile from the point of blood drawing till the point of infusion to the patient (Tullis et al 1959). The blood is drawn from the patient in a container which contains ACD solution added to it. It is also ensured that blood is drawn in a clean method with no requirement for giving a second puncture as this is an important protocol of platelet collection that blood should be drawn from one puncture. This blood is circulated through a tube of plastic which is maintained at a temperature of 4 degrees centigrade. The blood flows in this chamber and the residue that is left flows into a separate pool and this residue is basically the plasma which is devoid of red and white blood cells and contain only platelets. This platelet containing plasma is then passed through another chamber such that 10 ml of the fluid flows in every one minute. The platelets flow into a collector where they are maintained at 250 x gravity.

Professional Role Transition Assignment Example | Topics and Well Written Essays - 750 words

Professional Role Transition - Assignment Example Data sources that contained information related to the topic were also used during the research process. The studies presented evidences such as quoting those who were interviewed during the study. This showed that students were given equal opportunity to answer the questions directed at them. The studies also took a while to be completed. A slow research ensures that the researcher gains all the information required. Things tend to change with time and by conducting a research study within a long time, the researcher gets to record every change that may take place and also predict how the study will be in future. What I know about the journal is that it intends to understand the challenges that students face when transitioning into BN from LPN. The journal identifies all the challenges and helps to explain methods that can be used to curb them. The journals target LPN students who may be interested in studying BN. The study will help them understand the challenges they are bound to face. The journals also target other scholars that focus on studying the topic. These journals will give them a point of reference for their study. Educators in the institutions that the students enroll for BN are also a target audience for the study because they will have the knowledge of how to help the students adapt to the transition. Because of the challenges faced when transitioning from LPN to BN, students opt to turn to their mentors at their places of work to guide them. These mentors are in a great position to help them put into practice what they have studied. The journals have also helped explain why the LPNs choose to expand their knowledge. One of the reasons given by the participants is that at the work place, they are considered less of nursing practitioners and their opinions are not valued. Some of them had to endure being called Little Pretend Nurses instead of their real titles. For the students who have

Streak Plate And Viable Cell Count

Streak Plate And Viable Cell Count Aim and introduction should display insight into what the streak plate and viable cell count method are employed to achieve. They should also introduce MacConkey agar and how its selective and differential properties allow the characteristics of the test organism to be determined. Escherichia coli (E.coli) The aim of this experiment is to allow a certain bacterium to divide and multiply enabling us to view the bacteria in a single cell structure. E.coli is one bacterium that is good for such an experiment. E.coli can be said to be both bad and harmless, some E.coli bacteria have are highly toxic and can harm humans and animals. However, the majority of E.coli strains are relatively harmless with low toxicity. These harmless strains of E.coli are found naturally occurring in the human body, especially in areas such as the human intestines. Some E.coli can even benefit their hosts; they do this by producing specific vitamins. It is for reasons like the ones mentioned why E.coli is an appropriate bacteria to use for this experiment. Another reason is that E.coli bacterial cells have an average bacterial size of 2um; this can be seen under a light microscope. Other bacteria however may be even smaller and may require a larger microscope for viewing or even an electron microscope. Also the incubation period for E.coli to multiply and grow rapidly isnt very long and temperatures arent too high or too low. E.coli can be incubated overnight at 37oC and then stored at 4oC until its requirement. The technique use to manipulate and isolate the E. coli bacteria is known as the streak plate procedure. The technique was developed to allow bacteria to multiply and produce many colonies, during the incubation period, depending on the amount of bacteria present. Each colony will contain millions of bacteria cells derived from a single parent cell. (Talk in more detail about this procedure). We will be using this technique to allow the E. coli to multiply and divide splitting itself into colonies. The viable cell count, also known as viability count, is a method used to determine the number of living cells within a suspension, in this case E. coli. To obtain an understanding of how much E. coli cells are present in a sample this method must be put into action. (Expand) The MacConkey agar is specifically designed to allow gram negative bacteria to grow; its a recipe of many substances such as bile salts, sodium chloride proteose and many more. One of the properties of the MacConkey agar is its selective isolation and identification of bacteria; it is a medium that allows us to distinguish gram-negative bacteria. E. coli is a rod shaped gram negative bacteria, so using the MacConkey agar plate to multiply it would be appropriate, the agar will also cause the E. coli to change colour from pink to red, and this is an indication of gram negative bacteria present. A Nutrient agar is a growth medium used for the cultivation of bacteria, this specific agar remains solid even at high temperatures. The gram-stain technique was developed for viewing cells clearly under a microscope and to enable us to establish their structures. It is a very simple procedure of just adding 4 different substances accordingly, however one of these substances is toxic to humans therefore the procedure must be carried out in a fume hood. The gram stain method was introduced by It is important for scientists and medics to know the structure and function and identity of bacteria and viruses, it is for reasons like this why such experiments are carried out. Without such procedures so many bacteria and viruses wouldnt be known and could spread and become out of control. Methods: Explain why each procedure was done highlight key points state any deviation from protocol document any errors or difficulties you had with the technique Discuss the importance of aseptic technique and what steps could be taken to prevent contamination during manipulation of bacteria All methods were doing under aseptic conditions; the reason for this is to prevent contamination of the bacteria during its manipulation. Many errors could arise if aseptic conditions arent used, eventually resulting is wrote results. Streaking bacteria on MacConkey agar method: Prior to the experiment, an E. coli sample was made and given to during the practical. Risk assessment: Materials used: 10ml liquid culture of E. coli the bacteria sample to be use in this practical Sterile plastic loops used for transferring E.coli bacteria from one place to another uncontaminated. MacConkey agar plate used to allow E.coli bacteria to grow as it provides energy recourses and support Sharps bin for loops etc. these are used to keep the laboratory area as uncontaminated as possible and to make sure bacteria doesnt spread Marker pens and labels to label the plate Step 1: using a sterile plastic loop I touched a given sample of E. coli and streaked an inoculum onto a MacConkey agar plate in a specific pattern (see..). This plastic loop is then disposed of into the sharps bin. Step 2: using another sterile plastic loop, I created a run of parallel lines from the edge of the initial streaks Step 3: step 2 was repeated 2 more times with a new sterile loop used. Step 4: a final streak was made, creating a simple streak from the previous streaks into the centre of the plate. The picture below illustrates this. The MacConkey plate was then given to the technicians to incubate. It was important to dispose- on the plastic looks by placing them into the sharps bin because they are contaminated and if they touch any other surface it can lead to the spread of bacteria resulting in major contamination. Throughout this procedure plastic gloves and a lab-coat were worn, also to prevent contamination. Viable cell counts: Risk assessment Materials used: P1000 and P100 pipettes and tips used to transfer certain amounts of PBS etc. Three Nutrient agar plates 10ml sterile PBS buffer, used to maintain the pH Sterile plastic spreaders to spread the E.coli on the Nutrient agar plates Eight sterile bijoux bottles for dilutions To start the dilution, using a pipette I transferred 900 ul of diluents (PBS) in eight different sterile bijoux bottles. The PBS (phosphate buffered solution) solution is a commonly used buffer to maintain a pH; it is used in this practical because of its ability to aid biological research. After the PBS was placed into the labelled bottles, using a new sterile tip for the pipette I transferred 0.1ml of E.coli liquid culture sample (neat) into the first bottle (10-1). For the dilution to continue a new pipette tip was placed and 0.1ml of the 10-1 diluted E.coli was transferred to the 10-2 bottle, this process continued up till bottle 10-8. By doing so the E.coli will become more and more dilute within the different solutions, because less E.coli is being added each time. 10-5, 10-6 and 10-7 samples were then spread onto three different Nutrient agar plates using different sterile plastic spreaders so contamination wouldnt occur. This was done by placing 0.1ml of each dilution onto th e centre of the agar plate and then spreading it over with a sterile spreader. The agar plates were labelled and given to the technician for incubation. Gram Stain of bacteria from an isolated colony Risk assessment In order to stain the bacteria I selected an appropriate colony to stain, the colony must appear to be uncontaminated and its appearance must obviously look grown.. After this procedure is complete, the bacterial cells will be visible under a microscope. Materials needed: Bunsen burner used to heat-fix bacteria onto microscope slide Saline (PBS) emulsifier Light microscope to view bacterial cells Lens tissue to clean the lens Immersion oil for light microscope lens to allow better view at 100x magnification Stains for Gram stain method Before the bacteria can be modified to be viewed under the microscope clearly, the microscope glass slide must be cleaned to prevent contamination. After doing so a drop of sterile saline was placed onto the centre of the slide, the saline drop was placed because it can emulsify any bacterial colony that will be placed on top. To move some of the bacteria off the agar plate onto the slide, a sterile loop was used I touched the bacterial colony on the agar plate with the top of the loop and then spread the bacteria into the saline drop making it thin. Due to the moisture of the liquid I let the slide dry then used a Bunsen-burner to heat-fix the bacteria onto the slide by passing it through a few times then allowing it to cool. Heat-fixing was done so that during the staining the bacteria or wouldnt move or fall off. Once that was complete the slide was moved to a laboratory fume hood where the staining can take place, the follow 4 stage method was used: at first the bacteria sample on the slide was soaked in crystal violet for 30 seconds, after so it was rinsed with distilled water and drained. The second part is to soak the bacteria with gram iodine (mordant) for another 30 seconds then rinse with distilled water and drain it. Gram iodine is a toxic substance; it is for this particular reason why this part of the practical was carried out in a laboratory fume hood. Acetone decolouriser was then added for 10 second and the bacteria was again rinsed with distilled water and drained. The final part is to add Safranin, a counter stain, to the bacteria. It was placed on the bacteria for 30seconds and then the bacteria was further rinsed with distilled water, drained, blotted and allowed to dry. Substance Duration Further action Crystal violet (primary stain) 30 seconds Rinse with water drain Gram Iodine (Mordant) 30 seconds Rinse with water drain Acetone/alcohol (Decolouriser) 5-10 seconds Rinse with water drain Safranin (Counter stain) 30 seconds Rinse with water, drain, blot dry Stain was carried out in a laboratory fume hood due to the toxic gram iodine substance used. The transparent plastic shield of the fume hood was lowered so that only my hands were inside dealing with the chemical and biological substances. Gloves were worn during this procedure so that no stain would come into contact with the skin. When the slide was rinsed with water, it was rinsed gently with distilled water so that the bacteria are not shifted. After the staining was completed the sample can now be viewed under a light microscope and compared to other bacterial samples. The slide is placed on the stage with a drop of oil for immersion, the microscope is focused on 100x and the bacteria is viewed. Results: Should describe your findings in : prose/text, diagrams, tables and graphs which includes a description of growth characteristics and how successful your aseptic technique was MacConkey agar plate results: During the experiment there were no results to be noted as it was too early for anything to occur. After the agar plate containing the E.coli was incubated at 37oC and then stored at 4oC, its appearance was as expected. Colonies were separated, and as the streaks moved on less E.coli was present. The colonies were well distinctive and were round in their shape. The sample initially given was simply liquid, the result showed significant growth of this E.coli liquid into 3D structures. This indicates the growth of the bacteria in a fine way; the 3D structures appeared in a yellowing solid colour. Because the practical was conducted in aseptic techniques no contamination occurred. Aseptic techniques were successful in allowing me to produce accurate results. Viable cell counts My results: The colonies that appeared on the nutrient plate had a badge colour; visually they all appeared relatively same sized and volume. 10-5 10-6 10-7 10-8 TMTC 46 1 For the 10-7 the calculation for the number of bacteria in 1ml of the original culture is: (1107/0.1) x (X/1) [cross multiply] 0.1X = 1 x(1107) [divide by 0.1] Therefore X = 1.0108 For the 10-6 the calculation for the number of bacteria in 1ml of the original culture is: (46106/0.1) x (X/1) [cross multiply] 0.1X = 1 x(46106) [divide by 0.1] Therefore X = 4.6108 The number of bacteria present in 1ml of the 10-5 culture cannot be calculated as there was no value noted (TMTC). X= number of bacteria. The number of bacteria present in 1ml of 10-6 dilution is 4.6108 and in the 10-7 dilution culture is 1.0108. Class results: Pair number 10-5 10-6 10-7 10-8 1 TMTC 46 1 2 95 9 0 3 TMTC 52 9 4 TMTC 23 1 5 34 2 6 84 11 4 7 TMTC 24 18 8 2 2 26 9 102 6 0 10 19 3 3 11 TMTC 140 15 12 TMTC 57 9 13 61 12 0 14 8 6 1 15 195 51 4 16 55 27 3 17 TMTC 94 11 18 TMTC TMTC TMTC 19 TMTC TMTC TMTC 20 TMTC 113 18 Total 266 985 177 28 Average 66.5 57.9 9.8 7 Should describe your findings in : prose/text, diagrams, tables and graphs which includes a description of growth characteristics and how successful your aseptic technique was To determine the amount of bacteria within a culture a simple calculation must be done using my personal results for this experiment. There was no value for the 10-5 so this cannot be done. The result for 10-6 was 46, 46 x 10 = 460ml. To estimate the amount of E.coli present this is further multiplied by 106, therefore 460 x 106 = For the 10-7 result Æ’Â   7 x 10 = 10 Æ’Â   10 x 107 = However, I have selected some reasonable results from the table to calculate an average. Gram-stain of bacteria from an isolated colony: (view method number 3) Gram stains help us distinguish between microbial organisms, for example gram negative bacteria and gram positive bacteria. This method was developed to know the identity of bacteria present. (See procedure for The Gram Stain in the methods section). During step 5 of the Gram Strain Method above the following results were made when applying the four different substances: Substance Colour after stain Crystal violet (primary stain) Purple Gram Iodine (Mordant) Purple Acetone/alcohol (Decolouriser) Transparent (dye was washed off) Safranin (Counter stain) Reddish-pink The appearance of the E.coli bacteria under a microscope with 100x magnification was quite clear; it had a rod-like structure with a reddish-pink colour. The rods were all more or less the same size, however some were packed together and others were on their own. Discussion: Were the results the expected? Did the methods adopted achieve their aim? How the experiments could be improved. Include background information, critical evaluation of results Throughout all the experiments and procedures a lab-coat and gloves were worn to avoid skin contact with bacteria and harmful substances. Overall the aims were accomplished and the results were as predicted. MacConkey agar The colonies were expected to be in such a form, indicating that it was E.coli present and that it has rapidly multiplied into individual colonies. This further suggests that when E.coli is present under conditions where it could multiply, it multiplies by forming a round colony and expanding from there. However, some of the colonies were stuck together making it difficult to count the number of them present. What this means is that the growth of the bacteria was a success and the method adopted was accurate. The reason why some colonies were packed together may be the result of pressing too hard on the agar while streaking, with more streaking practice more accurate results can be obtained with colonies being on their own. The methods adopted for this practical achieved what was aimed for. After the incubation of the MacConkey agar plate the plate was stored for a week at a temperature of 4oC, this may have changed the appearance in colour and in shape of the formed colonies. Contam ination of the agar plate may have even occurred. An improvement to the experiment is to note down results straight after incubation is finished. Gram Stain results After analysing the microscope slide which contains the Gram Stained E.coli under the microscope its features were obvious. There were many average sized rods with a reddish-pink colour, some of these rods were packed together whist others were separated. Comparing this with another prepared sample of B.subtilis, the B.subtilis was a purple colour and has a longer and curved shape, like fine threads. However some again were packed together and others separated. The purple colour of the B.subtilis indicates that it is gram positive, and the pink colour of E.coli indicates its gram negative. When the Gram Stain method was applied to the B.subtilis it obviously stayed purple though out, with E.coli it will decolourise once the decolouriser is added. The gram stain method is highly effective and efficient when dealing with different bacteria; it helps identify them to a great extent. B.subtilis remains purple throughout the Gram Stain procedure, this itself can be an indication that it is a Gram positive bacteria. Bacterial cells have different types of cell walls, the gram negative and gram positive terms describe the nature of their structural differences. One of the important differences is that Gram positive bacteria have no outer membrane whereas Gram negative bacteria do, the purpose of this outer layer is to cover the peptidoglycan layer. When staining occurs the outer membrane of a gram positive bacterial cell wall becomes permanently stained as the strain can easily penetrate the thick peptidoglycan layer, so that if a decolouriser or distilled water is added the colour will remain purple. In the case of the gram negative bacterial cell wall the stain gets attached to the far outer membrane layer (lipopolysaccharide and protein), this layer decreases the penetration depth of the strain on the peptidoglycan, so the stain can be decolourised or removed. The diagrams below illustrate this. Gram positive: Æ’Â   Æ’Â   Æ’Â   Primary stain Mordant Decolourisation Counter-stain Note: colour remains the same throughout addition. Gram negative: Primary stain Mordant Decolourisation Counter-stain Note: colour changes Æ’Â   Æ’Â   Æ’Â   The aim of the Gram Stain method was confirm that the bacteria that was initially being dealt with was E.coli, after tests and results it confirmed that it was so the results were as expected and predicted. The methods used for this procedure were successful at achieving good results, however some can be altered. For example, the E.coli used for this experiment was used from experiment number one, not that that is a problem but when the E.coli was incubated over night and it had successfully multiplied it was stored at 4oC for quite a while (this experiment was carried out 1 week after the first one). This possible may have altered the activity of the E.coli and also its appearance. Many resources state that gram negative bacteria should have a pink colour after the counter-stain has been added and rinsed off. In this case the E.coli bacteria in this experiment had quite a dark pink colour which was really close to the colour red, this appearance of colour was visual both with the naked eye and under the microscope as individual bacterial cells. Viable cell counts As I predicted, the more dilute (10-8) solution will have less E.coli bacteria growing on its surface. As there were 20 different pairs doing the practical, and the dilutions were all done 20 times by different people, there is plenty room for error from contamination of inaccurate measurements. The 10-5 agar plates had many E.coli bacterial colonies growing on it, according to the results there was far too many bacteria that it was too many to count (TMTC). Gradually as the dilution increased the bacteria became less, 10-6 dilution had numbers ranging from 6-140. Obviously with such great difference within what is meant to be the same dilution there was some error/contamination present. The most obvious ones which had error are pair numbers 18 and 19 there was TMTC throughout (10-6, 10-7 and 10-8). What would be expected is that fewer bacteria should be present in the 10-8.

Personalities of The Cold War Essay

Who caused the cold war? Focus; the role of each personality in contributing to the cold warTime frame: 1945 to 1952Cold war- period of intense tension and mistrust, leading to competition and confrontations. Stand: both Stalin and Truman contributed to the cold war. The key personalities that contributed to the cold war are namely Stalin and Truman,both in office in US and USSR respectively. both were responsible as their personalities and level of experiences contributed to their policies made, which heightened tensions and thus caused the cold war. Personality traits like Stalin’s paranoia gave rise to his expansionist policy, which hardline Truman viewed as aggression and tried to counter it in the Truman Doctrine and Marshall plan. Truman’s low level of experience in dealing with Stalin also increased tensions and led to the cold war. Stalin was aggressive and protective of the USSR as he was a true hardline communist and believed that Russia had to stay strongly communist. However, the USSR was invaded thrice in no more than a century and also suffered civil war and intervention from anti-communist forces from 1918 to 1920, when communism as an ideology was at infancy. These anti-communist forces comprised of the West when they helped the Whites during the Bolshevik Revolution. From this, Stalin believed that the West wanted to destroy communism before communism became stronger. His mistrust grew as he became paranoid and thought of the West as a potential security threat this was because This prompted his embarking on salami tactics ( an expansionist policy) so that Communism would remain strong in Eastern Europe. Gradually the Russians began to systematically interfere in the countries in Eastern Europe to set up pro-communist governments, in countries like Poland, Hungary, Bulgaria, Albania and Romania. Stalin felt that his actions were justified for the defense of communism, and that salami tactics were absolutely necessary. He did not realize that his actions had frightened the West. What he failed to consider was that the west was not interested in destroying communism but was looking more towards post war cooperation. The  west showed this through the decisions made at the post-war conferences when Russia was allowed to take reparations from Germany and it was allowed to benefit from the loans from the west, known as the Lend Lease. His paranoia in embarking on the expansionist policy ( comprising Salami tactics) when juxtaposed with Truman’s hardline views, only served to heighten suspicion and tensions, leading to the Cold war. Similarly, Truman became suspicious of the USSR’s intent towards eastern Europe. He was a hardline president who stood firmly against Communism, and was intolerant of the needs of the USSR . Truman saw Stalin’s actions as a breach of the Declaration of Eastern Europe where Stalin had promised to allow countries like Poland free elections but failed to do so, instead carrying out the reverse and forcing communist governments in these countries through rigged electionsTruman, with his hardline view that communism was bad, viewed Stalin’s moves as being remarkably similar to Hitler’s salami tactics, and that the USSR was embarking on aggression. In addition he felt that if he did not stop Stalin, Stalin would think that Eastern Europe was his for the taking, and capitalism would perish. This in turn led to the formation of the Truman Doctrine and Marshall plan which would serve to increase tensions (elaborated below)Secondly, the Truman Doctrine and the Marsh all Plan. The West formulated the Truman Doctrine and the Marshall plan to contain communism in Greece and Turkey. Communists were trying to overthrow the monarchy but british troops who restored the monarchy in the past were feeling the strain of supporting it against the communists. The british prime minister appealed to the USA and Truman announced that it would † support free peoples who are resisting subjugation by armed minorities or by outside pressure’ and Greece received massive amounts of aid and the communists were defeated. The funds of the Truman doctrine were obtained through Congress where Truman portrayed the situation in Greece and Turkey as part of the global communist threat. Truman’s low level of experience prevented him from realizing that Stalin would see the Truman doctrine as an attempt to subvert them. In other words, he did not deal with stalin before, and did not see that his portrayal was excessively confrontational and would  serve to heighten Stalin’s paranoia and escalate his mistrust towards the West. Similarly, the Marshall plan was formulated to facilitate economic recovery in Eastern Europe. By September, 16 nations had drawn up a joint plan for using American aid and in total over 13000 million dollars of Marshall Aid was given to western European countries. However, the west did not consider the impact that the Marshall plan would have on how the USSR viewed them. Truman’s low level of experience with dealing with the Stalin prevented him from seeing that the Stalin was sensitive about USSR’s economic status (Stalin refused to ratify the the Bretton Woods agreement so that the West would not realize how economically weak the USSR was in 1945. The Bretton woods agreement was a system to acquire international currency stabilization which required foreign access to sensitive economic data. Stalin viewed the policy as ‘dollar imperialism† and as a blatant American device for gaining control of western Europe, and made all Russian states reject the offer. The Truman doctrine and the Marshall plan would eventually prompt Stalin( along with his paranoia) to come up with the Cominform and the Comecon which served to unify all Eastern European satellite states. Truman’s low level of experience in coming up with the Truman Doctrine and the Marshall plan, when juxtaposed with Stalin’s paranoia, fueled further consolidation of power by Stalin, which escalated tensions on both the USA and the USSR and caused the Cold war. Biblography: Richard Crockett, the fifty years war: The United States and the Soviet Union in World Politics, 1945-1991Gaddis, John Lewis. We now know: Rethinking Cold war History. US: Oxford University Press.